An improved fluorogenic NAD(P)+ detection method using 2-acetylbenzofuran: its origin and application.

نویسندگان

  • Takashi Moriya
  • Ayano Kawamata
  • Yusuke Takahashi
  • Yoshiharu Iwabuchi
  • Naoki Kanoh
چکیده

We have developed a fluorogenic NAD(P)(+) detection method using 2-acetylbenzofuran. The reaction of NAD(P)(+) with 2-acetylbenzofuran produced a fluorescent product, allowing the highly-sensitive and quick detection of NAD(P)(+). This method was successfully applied to the detection of P450 substrates in the microtiter-plate format.

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عنوان ژورنال:
  • Chemical communications

دوره 49 98  شماره 

صفحات  -

تاریخ انتشار 2013