An improved fluorogenic NAD(P)+ detection method using 2-acetylbenzofuran: its origin and application.
نویسندگان
چکیده
We have developed a fluorogenic NAD(P)(+) detection method using 2-acetylbenzofuran. The reaction of NAD(P)(+) with 2-acetylbenzofuran produced a fluorescent product, allowing the highly-sensitive and quick detection of NAD(P)(+). This method was successfully applied to the detection of P450 substrates in the microtiter-plate format.
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عنوان ژورنال:
- Chemical communications
دوره 49 98 شماره
صفحات -
تاریخ انتشار 2013